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FAM171B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM171B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM171B antibodies →
$504.00each
Catalog Number: LS-G104912-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM171B (NM_177454), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM171B | KIAA1946 | Protein FAM171B
Origin Species
Human
NCBI GenBank
NM_177454
SwissProt
Q6P995

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARLCRRVPC TLLLGLAVVL LKARLVPAAA RAELSRSDLS LIQQQQQQQQ QQQQQQQQKQ
61LEEAEEERTE VPGATSTLTV PVSVFMLKVQ VNDIISRQYL SQAVVEVFVN YTKTNSTVTK
121SNGAVLIKVP YKLGLSLTII AYKDGYVLTP LPWKTRRMPI YSSVTLSLFP QSQANIWLFE
181DTVLITGKLA DAKSQPSVQF SKALIKLPDN HHISNVTGYL TVLQQFLKVD NFLHTTGITL
241NKPGFENIEL TPLAAICVKI YSGGKELKVN GSIQVSLPLL RLNDISAGDR IPAWTFDMNT
301GAWINHGRGM VKEHNNHLIW TYDAPHLGYW IAAPLPGTRG SGINEDSKDI TAYHTVFLTA
361ILGGTIVIVI GFFAVLLCYC RDKCGTPQKR ERNITKLEVL KRDQTTSTTH INHISTVKVA
421LKAEDKSQLF NAKNSSYSPQ KKEPSKAETE ERVSMVKTRD DFKIYNEDVS FLSVNQNNYS
481RNPTQSLEPN VGSKQPKHIN NNLSSSLGDA QDEKRYLTGN EEAYGRSHIP EQLMHIYSQP
541IAILQTSDLF STPEQLHTAK SATLPRKGQL VYGQLMEPVN RENFTQTLPK MPIHSHAQPP
601DAREEDIILE GQQSLPSQAS DWSRYSSSLL ESVSVPGTLN EAVVMTPFSS ELQGISEQTL
661LELSKGKPSP HPRAWFVSLD GKPVAQVRHS FIDLKKGKRT QSNDTSLDSG VDMNELHSSR
721KLEREKTFIK SMHQPKILYL EDLDLSSSES GTTVCSPEDP ALRHILDGGS GVIMEHPGEE
781SPGRKSTVED FEANTSPTKR RGRPPLAKRD SKTNIWKKRE ERPLIPIN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

828 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.