Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM164A / CGI-62 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112924-100
Catalog Number: LS-G112924
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CGI 62(ZC2HC1A) (NM_016010), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C8orf70 | CGI-62 | FAM164A | Protein FAM164A | ZC2HC1A
- Origin Species
- Human
- NCBI GenBank
- NM_016010
- SwissProt
- Q96GY0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGLEENGGA VQVGELLPCK ICGRTFFPVA LKKHGPICQK TATKKRKTFD SSRQRAEGTD61IPTVKPLKPR PEPPKKPSNW RRKHEEFIAT IRAAKGLDQA LKEGGKLPPP PPPSYDPDYI121QCPYCQRRFN ENAADRHINF CKEQAARISN KGKFSTDTKG KPTSRTQVYK PPALKKSNSP181GTASSGSSRL PQPSGAGKTV VGVPSGKVSS SSSSLGNKLQ TLSPSHKGIA APHAGANVKP241RNSTPPSLAR NPAPGVLTNK RKTYTESYIA RPDGDCASSL NGGNIKGIEG HSPGNLPKFC301HECGTKYPVE WAKFCCECGI RRMILTRTRP LEQKLISEED LAANDILDYK DDDDKV
356 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.