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FAM161B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM161B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM161B antibodies →
$504.00each
Catalog Number: LS-G106209-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM161B (NM_152445), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf44 | C14_5547 | FAM161B | Protein FAM161B
Origin Species
Human
NCBI GenBank
AA428461
SwissProt
Q96MY7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTVGRPEGAP GGAEGSRQIF PPESFADTEA GEELSGDGLV LPRASKLDEF LSPEEEIDST
61SDSTGSIYQN LQELKQKGRW CLLESLFQSD PESDENLSED EEDLESFFQD KDRGMVQVQC
121PQALRCGSTR RCSSLNNLPS NIPRPQTQPP SGSRPPSQHR SVSSWASSIT VPRPFRMTLR
181EARKKAEWLG SPASFEQERQ RAQRQGEEEA ECHRQFRAQP VPAHVYLPLY QEIMERSEAR
241RQAGIQKRKE LLLSSLKPFS FLEKEEQLKE AARQRDLAAT AEAKISKQKA TRRIPKSILE
301PALGDKLQEA ELFRKIRIQM RALDMLQMAS SPIASSSNRA NPQPRTATRT QQEKLGFLHT
361NFRFQPRVNP VVPDYEGLYK AFQRRAAKRR ETQEATRNKP FLLRTANLRH PQRPCDAATT
421GRRQDSPQPP ATPLPRSRSL SGLASLSANT LPVHITDATR KRESAVRSAL EKKNKADESI
481QWLEIHKKKS QAMSKSVTLR AKAMDPHKSL EEVFKAKLKE NRNNDRKRAK EYKKELEEMK
541QRIQTRPYLF EQVAKDLAKK EAEQWYLDTL KQAGLEEDFV RNKGQGTRAV QEKETKIKDF
601PRFQETTKLS IRDPEQGLEG SLEQPASPRK VLEELSHQSP ENLVSLA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

647 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.