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FAM161A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM161A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM161A antibodies →
$504.00each
Catalog Number: LS-G129080-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM161A (NM_032180), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM161A | Protein FAM161A | RP28
Origin Species
Human
NCBI GenBank
AK023367
SwissProt
Q3B820

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKLEKMYQD KLHLKEVQPV VIREDSLSDS SRSVSEKNSY HPVSLMTSFS EPDLGQSSSL
61YVSSSEEELP NLEKEYPRKN RMMTYAKELI NNMWTDFCVE DYIRCKDTGF HAAEKRRKKR
121KEWVPTITVP EPFQMMIREQ KKKEESMKSK SDIEMVHKAL KKQEEDPEYK KKFRANPVPA
181SVFLPLYHDL VKQKEERRRS LKEKSKEALL ASQKPFKFIA REEQKRAARE KQLRDFLKYK
241KKTNRFKARP IPRSTYGSTT NDKLKEEELY RNLRTQLRAQ EHLQNSSPLP CRSACGCRNP
301RCPEQAVKLK CKHKVRCPTP DFEDLPERYQ KHLSEHKSPK LLTVCKPFDL HASPHASIKR
361EKILADIEAD EENLKETRWP YLSPRRKSPV RCAGVNPVPC NCNPPVPTVS SRGREQAVRK
421SEKERMREYQ RELEEREEKL KKRPLLFERV AQKNARMAAE KHYSNTLKAL GISDEFVSKK
481GQSGKVLEYF NNQETKSVTE DKESFNEEEK IEERENGEEN YFIDTNSQDS YKEKDEANEE
541SEEEKSVEES H
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

551 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.