Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM155B / TMEM28 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113052-100
Catalog Number: LS-G113052
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM155B (NM_015686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BB57D9.1 | BB57D9.1 (TED protein) | CXorf63 | TED | Transmembrane protein 28 | Transmembrane protein FAM155B | Protein TED | FAM155B | TMEM28
- Origin Species
- Human
- NCBI GenBank
- NM_015686
- SwissProt
- O75949
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFRGAWMWPG KDAAALTICC CCCCWAPRPS DKPCADSERA QRWRLSLASL LFFTVLLADH61LWLCAGARPR ARELSSAMRP PWGAGRERQP VPPRAVLPLP PPPPGEPSAP PGTCGPRYSN121LTKAAPAAGS RPVCGGVPEP TGLDAACTKL QSLQRLFEPT TPAPPLRPPD SLSRAPAEFP181SAKKNLLKGH FRNFTLSFCD TYTVWDLLLG MDRPDSLDCS LDTLMGDLLA VVASPGSGAW241EACSNCIEAY QRLDRHAQEK YDEFDLVLHK YLQAEEYSIR SCTKGCKAVY KAWLCSEYFS301VTQQECQRWV PCKQYCLEVQ TRCPFILPDN EEMVYGGLPG FICTGLLDTS PKRLETKCCD361VQWVSCEAKK KKFKESEAPK THQQQFHHSY FHHYHQQYHH YHPHHDPPGR VSNKPALLPV421SGGSRLSPSR IRLCVLVLML LHTVVSFSSN QGGGGLGLET LPALEEGLTR EETRTRPLEQKLISEEDLAANDILDYKDDDDKV
472 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.