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FAM151A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM151A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM151A antibodies →
$504.00each
Catalog Number: LS-G104923-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM151A (NM_176782), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hyporthetical protein MGC27169 | FAM151A | Protein FAM151A | C1orf179
Origin Species
Human
SwissProt
Q8WW52

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVCREQLSKN QVKWVFAGIT CVSVVVIAAI VLAITLRRPG CELEACSPDA DMLDYLLSLG
61QISRRDALEV TWYHAANSKK AMTAALDSNI TVLEADVNVE GLGTANETGV PIMAHPPTTY
121SDNTLEQWLD AVLGSSQKGI KLDFKNIKAV GPSLDLLRQL TEEGKVRRPI WINADILKGP
181NMLISTEVNA TQFLALVQEK YPKATLSPGW TTFYMSTSPN RTYTQAMVEK MHELVGGVPQ
241RVTFPVRSSM VRAAWPHFSW LLSQSERYSL TLWQAASDPM SVEDLLYVRD NTAVHQVYYD
301IFEPLLSQFK QLALNATRKP MYYTGGSLIP LLQLPGDDGL NVEWLVPDVQ GSGKTATMTL
361PDTEGMILLN TGLEGTVAEN PVPIVHTPSG NILTLESCLQ QLATHPGHWG IHLQIVEPAA
421LRPSLALLAR LSSLGLLHWP VWVGAKISHG SFSVPGHVAG RELLTAVAEV FPHVTVAPGW
481PEEVLGSGYR EQLLTDMLEL CQGLWQPVSF QMQAMLLGHS TAGAIGRLLA SSPRATVTVE
541HNPAGGDYAS VRTALLAARA VDRTRVYYRL PQGYHKDLLA HVGRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

585 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.