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FAM149A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM149A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM149A antibodies →
$725.00each
Catalog Number: LS-G113238-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM149A (NM_015398), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP564J102 | FAM149A | Protein FAM149A | MSTP119 | MST119
Origin Species
Human
NCBI GenBank
NM_001006655
SwissProt
A5PLN7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MLFEGKVNPQ TQSLLAECGE WTRRSLHLRV LGRQLILPTD KGVQHFQGST PASAVHRPPL
61SACGHSSNIR ELCISGSQIV PAALSASALP GPDDTGVADL TARSSLEEEV YHVDGKIEEY
121FAFDRKEDDD ECLEQKPAQP GRKWRKLGLP PVSPRDCVKD AVAAEVFDHV WTNMVELLEE
181LIRKHWETTL TEGKKQRETL KVAGNRFPHV LVPHAHADGA SGPPSGHAEA HGISLASRLN
241PPQIHHFSSS FYSDMNGVMT IQAKPLQRRP AYFADRTQNE KEDKASGGGA GALSSAPHRL
301GRASDTHGLS PSAKKTPVPW RLPSLASDSQ RLKTPNIYSD EVLRGTKLPT GVDHMASPLV
361QTSRSRFPPL VTETRGQNTA VPGCRLVSYR GRHLQNRVLS AMPDGTERSR LRERTATLER
421LSRPSTTHTF RQSDTPRKSS LTQMEFAAHT WTGQSILTGS QYVPKSFQRT TLTFKRRFQV
481TS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

482 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.