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FAM13B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM13B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM13B antibodies →
$725.00each
Catalog Number: LS-G118974-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM13B1(FAM13B) (NM_001101801), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C5orf5 | FAM13B1 | GAP-like protein N61 | N61 | Protein FAM13B | ARHGAP49 | FAM13B | KHCHP
Origin Species
Human
NCBI GenBank
NM_016603
SwissProt
Q9NYF5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQLSQDYNNE DEFGRKLRFL LQQLPPVNYS LLKFLCRFLA NVASHHEEIW SANSLAAVFG
61PDVFHIYTDV EDMKEQEIVS RIMAGLLENY YEFFENEEED FSSNDLSSIT EQVNELSEEE
121EEDEKLEHIE ELPEEGAEKS NDMPEVVQLR MTENILESNS VTATSTHISP ISILPASTDI
181LERTIRAAVE QHLFDLQSSI DHDLKNLQQQ SVVCNNEAES IHCDGEGSNN QIDIADDIIN
241ASESNRDCSK PVASTNLDNE AMQQDCVFEN EENTQSVGIL LEPCSDRGDS EDGCLEREEY
301LLFDSDKLSH LILDSSSKIC DLNANTESEV PGGQSVGVQG EAACVSIPHL DLKNVSDGDK
361WEASCPITFP LIDFKTMHLQ RDGEEPFPAF KSWQEDSESG EAQLSPQAGR MNHHPLEEDC
421PPVLSHRSLD FGQSQRFLHD PEKLDSSSKA LSFTRIRRSS FSSKDEKRED RTPYQLVKKL
481QKKIRQFEEQ FERERNSKPS YSDIAANPKV LKWMTELTKL RKQIKDAKHK NSDGEFVPQT
541RPRSNTLPKS FGSSLDHEDE ENEDEPKVIQ KEKKPSKEAT LELILKRLKE KRIERCLPED
601IKVTKEERHI VKPLYDRYRL VKQMLTRASI TPVLGSPSTK RRGQMLQPII EGETAHFFEE
661IKEEEEDGVN LSSELGDMLK TAVQVQSSLE NSESDVEENQ EKLALDLRLS SSRAASMPEL
721LEQLWKARAE KKKLRKTLRE FEEAFYQQNG RNAQKEDRVP VLEEYREYKK IKAKLRLLEV
781LISKQDSSKS I
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

791 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.