Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM13A1 / KIAA0914 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113621-100
Catalog Number: LS-G113621
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM13A (NM_014883), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGAP48 | FAM13A | FAM13A1 | KIAA0914 | FAM13A1_v2 protein | Protein FAM13A
- Origin Species
- Human
- NCBI GenBank
- AB020721
- SwissProt
- O94988
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 116.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAGALAICQ SKAAVRLKED MKKIVAVPLN EQKDFTYQKL FGVSLQELER QGLTENGIPA61VVWNIVEYLT QHGLTQEGLF RVNGNVKVVE QLRLKFESGV PVELGKDGDV CSAASLLKLF121LRELPDSLIT SALQPRFIQL FQDGRNDVQE SSLRDLIKEL PDTHYCLLKY LCQFLTKVAK181HHVQNRMNVH NLATVFGPNC FHVPPGLEGM KEQDLCNKIM AKILENYNTL FEVEYTENDH241LRCENLARLI IVKEVYYKNS LPILLTRGLE RDMPKPPPKT KIPKSRSEGS IQAHRVLQPE301LSDGIPQLSL RLSYRKACLE DMNSAEGAIS AKLVPSSQED ERPLSPFYLS AHVPQVSNVS361ATGELLERTI RSAVEQHLFD VNNSGGQSSE DSESGTLSAS SATSARQRRR QSKEQDEVRH421GRDKGLINKE NTPSGFNHLD DCILNTQEVE KVHKNTFGCA GERSKPKRQK SSTKLSELHD481NQDGLVNMES LNSTRSHERT GPDDFEWMSD ERKGNEKDGG HTQHFESPTM KIQEHPSLSD541TKQQRNQDAG DQEESFVSEV PQSDLTALCD EKNWEEPIPA FSSWQRENSD SDEAHLSPQA601GRLIRQLLDE DSDPMLSPRF YAYGQSRQYL DDTEVPPSPP NSHSFMRRRS SSLGSYDDEQ661EDLTPAQLTR RIQSLKKKIR KFEDRFEEEK KYRPSHSDKA ANPEVLKWTN DLAKFRRQLK721ESKLKISEED LTPRMRQRSN TLPKSFGSQL EKEDEKKQEL VDKAIKPSVE ATLESIQRKL781QEKRAESSRP EDIKDMTKDQ IANEKVALQK ALLYYESIHG RPVTKNERQV MKPLYDRYRL841VKQILSRANT IPIIGSPSSK RRSPLLQPII EGETASFFKE IKEEEEGSED DSNVKPDFMV901TLKTDFSARC FLDQFEDDAD GFISPMDDKI PSKCSQDTGL SNLHAASIPE LLEHLQEMRE961EKKRIRKKLR DFEDNFFRQN GRNVQKEDRT PMAEEYSEYK HIKAKLRLLE VLISKRDTDS1021KSMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1023 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.