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FAM134A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM134A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM134A antibodies →
$725.00each
Catalog Number: LS-G110017-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM134A (NM_024293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2orf17 | FAM134A | Protein FAM134A
Origin Species
Human
NCBI GenBank
NM_024293
SwissProt
Q8NC44

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASGGGGGNT GAGGGPGMGL SLGLGLGLSL GMSEATSEAE EEAATAEAVG RLATTLWLRL
61RGWEAVLAAA QRLLVWEKPL HSLVTAAALN GLFWLLSSSS LRPFFLLSVS LLAYFLLDLW
121QPRFLPDVSA SSPEEPHSDS EGAGSGARPH LLSVPELCRY LAESWLTFQI HLQELLQYKR
181QNPAQFCVRV CSGCAVLAVL GHYVPGIMIS YIVLLSILLW PLVVYHELIQ RMYTRLEPLL
241MQLDYSMKAE ANALHHKHDK RKRQGKNAPP GGDEPLAETE SESEAELAGF SPVVDVKKTA
301LALAITDSEL SDEEASILES GGFSVSRATT PQLTDVSEDL DQQSLPSEPE ETLSRDLGEG
361EEGELAPPED LLGRPQALSR QALDSEEEEE DVAAKETLLR LSSPLHFVNT HFNGAGSPQD
421GVKCSPGGPV ETLSPETVSG GLTALPGTLS PPLCLVGSDP APSPSILPPV PQDSPQPLPA
481PEEEEALTTE DFELLDQGEL EQLNAELGLE PETPPKPPDA PPLGPDIHSL VQSDQEAQAV
541AEP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.