Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM129A / NIBAN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108069-100
Catalog Number: LS-G108069
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM129A (NM_052966), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GIG39 | FAM129A | NIBAN | Protein FAM129A | Protein Niban | C1orf24
- Origin Species
- Human
- NCBI GenBank
- AF288391
- SwissProt
- Q9BZQ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGSASSQLD EGKCAYIRGK TEAAIKNFSP YYSRQYSVAF CNHVRTEVEQ QRDLTSQFLK61TKPPLAPGTI LYEAELSQFS EDIKKWKERY VVVKNDYAVE SYENKEAYQR GAAPKCRILP121AGGKVLTSED EYNLLSDRHF PDPLASSEKE NTQPFVVLPK EFPVYLWQPF FRHGYFCFHE181AADQKRFSAL LSDCVRHLNH DYMKQMTFEA QAFLEAVQFF RQEKGHYGSW EMITGDEIQI241LSNLVMEELL PTLQTDLLPK MKGKKNDRKR TWLGLLEEAY TLVQHQVSEG LSALKEECRA301LTKGLEGTIR SDMDQIVNSK NYLIGKIKAM VAQPAEKSCL ESVQPFLASI LEELMGPVSS361GFSEVRVLFE KEVNEVSQNF QTTKDSVQLK EHLDRLMNLP LHSVKMEPCY TKVNLLHERL421QDLKSRFRFP HIDLVVQRTQ NYMQELMENA VFTFEQLLSP HLQGEASKTA VAIEKVKLRV481LKQYDYDSST IRKKIFQEAL VQITLPTVQK ALASTCKPEL QKYEQFIFAD HTNMIHVENV541YEEILHQILL DETLKVIKEA AILKKHNLFE DNMALPSESV SSLTDLKPPT GSNQASPARR601ASAILPGVLG SETLSNEVFQ ESEEEKQPEV PSSLAKGESL SLPGPSPPPD GTEQVIISRV661DDPVVNPVAT EDTAGLPGTC SSELEFGGTL EDEEPAQEEP EPITASGSLK ALRKLLTASV721EVPVDSAPVM EEDTNGESHV PQENEEEEEK EPSQAAAIHP DNCEESEVSE REAQPPCPEA781HGEELGGFPE VGSPASPPAS GGLTEEPLGP MEGELPGEAC TLTAHEGRGG KCTEEGDASQ841QEGCTLGSDP ICLSESQVSE EQEEMGGQSS AAQATASVNA EEIKVARIHE CQWVVEDAPN901PDVLLSHKDD VKEGEGGQES FPELPSEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
928 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.