LSBio
FAM126B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM126B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM126B antibodies →
$504.00each
Catalog Number: LS-G105167-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM126B (NM_173822), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_173822

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGTDRCVVE EWLSEFKALP DTQITSYAAT LHRKKTLVPA LYKVIQDSNN ELLEPVCHQL
61FELYRSSEVR LKRFTLQFLP ELMWVYLRLT VSRDRQSNGC IEALLLGIYN LEIADKDGNN
121KVLSFTIPSL SKPSIYHEPS TIGSMALTEG ALCQHDLIRV VYSDLHPQRE TFTAQNRFEV
181LSFLMLCYNS AIVYMPASSY QSLCRMGSRV CVSGFPRQHE KHWKELCGRI VLDPEFMVQL
241LTGVYYAMYN GQWDLGQEVL DDIIYRAQLE LFSQPLLVAN AMKNSLPFDA PDSTQEGQKV
301LKVEVTPTVP RISRTAITTA SIRRHRWRRE GAEGVNGGEE SVNLNDADEG FSSGASLSSQ
361PIGTKPSSSS QRGSLRKVAT GRSAKDKETA SAIKSSESPR DSVVRKQYVQ QPTDLSVDSV
421ELTPMKKHLS LPAGQVVPKI NSLSLIRTAS ASSSKSFDYV NGSQASTSIG VGTEGGTNLA
481ANNANRYSTV SLQEDRLGQA GEGKELLSPG APLTKQSRSP SFNMQLISQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

530 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.