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FAM124A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM124A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM124A antibodies →
$725.00each
Catalog Number: LS-G106795-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM124A (NM_145019), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM124A | Protein FAM124A
Origin Species
Human
SwissProt
Q86V42

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPKAGGGGE EDDCVDSGAE TGGSDYSHLS STSNGFSTWR MPCCLFLYCT HGDKEKGRWS
61LQVEHTWPSG ELSVEEAQDP FLVSIHIIAD PGESQPLQEA IDNVLAWIHP DLPLFRVSER
121RASRRRRKPP KGAQPALAVV LFLQEEYGEE QILQLHRTLQ QPPWRHHHTE QVHGRFLPYL
181PCSQDFFTLA PGTPLWAIRP VHYGKEIVRF TVYCRYDNYA DSLRFYQLIL RRNPSQKKAD
241FCIFPIFSNL DVDIQFSLKR LPCDQCPVPT DSSVLEFRVR DIGELVPLLP NPCSPISEGR
301WQTEDHDGNK ILLQAQRVHK KFPKPGRVHH ASEKKRHSTP LPSTAVPSHT PGSSQQSPLN
361SPHPGPIRTG LPPGHQQEFA GRANSTPNPP WSFQRSKSLF CLPTGGPSLA SSAEPQWFSN
421TGAPGHRASE WRHGHLLSID DLEGAQETDV DTGLRLSSSD LSVVSAYSAP SRFCSTVETP
481LPSERCSSHW AAHKDSREGP LPTVSRVTTE ASWASLPFFT KRSSSSSATA RAAPPAPSTS
541TLTDSSPQLP CDTPKVKQTD GDMPPPPGSA GPGDNDMEEF YI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.