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FAM116A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM116A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DENND6A antibodies →
$504.00each
Catalog Number: LS-G106083-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM116A(DENND6A) (NM_152678), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AFI1A | DENND6A | FAM116A | DENN/MADD domain containing 6A
Origin Species
Human
SwissProt
Q8IWF6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALRGPAGLG PGSRRPLDEA VAGAEGREAP ALVAAGGAPE DDEEDDGRGR GLLRWDSFSA
61WLHCVCVVGF DLELGQAVEV IYPQHSKLTD REKTNICYLS FPDSNSGCLG DTQFCFRFRQ
121SSGRRVSLHC LLDQFDKDLP VYLKKDPAYF YGYVYFRQVR DKTLKRGYFQ KSLVLISKLP
181YIHFFHTVLK QIAPEYFEKN EPYLEAACND VDRWPAPVPG KTLHLPIMGV VMKVRIPTCH
241DKPGTTQIVQ LTQQVDTNIS VILPTVHEVD IFRCFCPVFL HSQMLWELVL LGEPLVVMAP
301SPSESSETVL ALVNCISPLK YFSDFRPYFT IHDSEFKEYT TRTQAPPSVI LGVTNPFFAK
361TLQHWPHIIR IGDLKPTGEI PKQVKVKKLK NLKTLDSKPG VYTSYKPYLN RDEEIIKQLQ
421KGVQQKRPSE AQSVILRRYF LELTQSFIIP LERYVASLMP LQKSISPWKS PPQLRQFLPE
481EFMKTLEKTG PQLTSRIKGD WIGLYRHFLK SPNFDGWFKT RRKEMTQKLE ALHLEALCEE
541DLLLWIQKHT EVETVDLVLK LKNKLLQADR EHLPVKPDTM EKLRTHIDAI ILALPEDLQG
601ILLKTGMT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

608 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.