Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM114A2 / C5orf3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111632-100
Catalog Number: LS-G111632
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM114A2 (NM_018691), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_018691
- SwissProt
- Q9NRY5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDKDDIETP LLTEAAPILE DGNCEPAKNS ESVDQGAKPE SKSEPVVSTR KRPETKPSSD61LETSKVLPIQ DNVSKDVPQT RWGYWGSWGK SILSSASATV ATVGQGISNV IEKAETSLGI121PSPSEISTEV KYVAGETNAK ENENSSPVAG AFGVFSTIST AVQSTGKSVI SGGLDALEFI181GKKTMDVIAE GDPGFKRTKG LMNRNATLSQ VLREAKEKEE IRTSNEVTVE TDKKTHYGLL241FDEFQGLSHL EALEMLSQES EIKVKSILNS LSGEELETLK VELEQLKETF SLAEFCEEEE301EEKKGDEDFT KDITELFSQL HVSSKPEKLA RARNTAHEWI RKSLTKPLAE NEEGEKQSEA361ENTEQVNKNS IEDIHAFAIR SLAELTACSI ELFHKTAALV LHGRKQEVTA IERSQTLSQM421TIVLCKELSS LSKEFTTCLT TAGVKEMADV LNPLITAVFL EASNSASYIQ DAFQLLLPVL481EISLIENKIE SHRHELQGQK PLLEHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
505 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.