Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM114A1 / Noxp20 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107362-100
Catalog Number: LS-G107362
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM114A1 (NM_138389), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_138389
- SwissProt
- Q9D281
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDDAGDTLA TGDKAEVTEM PNSDSLPEDA EVHCDSAAVS HEPTPADPRG EGHENAAVQG61AGAAAIGPPV QPQDANALEP PLNRDVTEDT LAECIDSVSL EAEPRSEIPL QEQNYPAVDS121PPSGGGWAGW GSWGKSLLSS ASATVGHGLT AVKEKAGATL RIHGVNSGSS EGAQPNTENG181VPEITDAATD QGPAESPPTS PSSASRGMLS AITNVVQNTG KSVLTGGLDA LEFIGKKTMN241VLAESDPGFK RTKTLMERTV SLSQMLREAK EKEKQRLAQQ LTMERTAHYG MLFDEYQGLS301HLEALEILSN ESESKVQSFL ASLDGEKLEL LKNDLISIKD IFAAKELENE ENQEEQGLEE361KGEEFARMLT ELLFELHVAA TPDKLNKAMK RAHDWVEEDQ TVVSVDVAKV SEEETKKEEK421EEKSQDPQED KKEEKKTKTI EEVYMSSIES LAEVTARCIE QLHKVAELIL HGQEEEKPAQ481DQAKVLIKLT TAMCNEVASL SKKFTNSLTT VGSNKKAEVL NPMISSVLLE GCNSTTYIQD541AFQLLLPVLQ VSHIQTSCLK AQPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
563 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.