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FAM111B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM111B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM111B antibodies →
$725.00each
Catalog Number: LS-G103400-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM111B (NM_198947), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CANP | Protein FAM111B | FAM111B
Origin Species
Human
SwissProt
Q6SJ93

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSMKTEENK SFSAMEDDQR TRPEVSKDTV MKQTHADTPV DHCLSGIRKC SSTFKLKSEV
61NKHETALEMQ NPNLNNKECC FTFTLNGNSR KLDRSVFTAY GKPSESIYSA LSANDYFSER
121IKNQFNKNII VYEEKTIDGH INLGMPLKCL PSDSHFKITF GQRKSSKEDG HILRQCENPN
181MECILFHVVA IGRTRKKIVK INELHEKGSK LCIYALKGET IEGALCKDGR FRSDIGEFEW
241KLKEGHKKIY GKQSMVDEVS GKVLEMDISK KKALQQKDIH KKIKQNESAT DEINHQSLIQ
301SKKKVHKPKK DGETKDVEHS REQILPPQDL SHYIKDKTRQ TIPRIRNYYF CSLPRKYRQI
361NSQVRRRPHL GRRYAINLDV QKEAINLLKN YQTLNEAIMH QYPNFKEEAQ WVRKYFREEQ
421KRMNLSPAKQ FNIYKKDFGK MTANSVSVAT CEQLTYYSKS VGFMQWDNNG NTGNATCFVF
481NGGYIFTCRH VVHLMVGKNT HPSLWPDIIS KCAKVTFTYT EFCPTPDNWF SIEPWLKVSN
541ENLDYAILKL KENGNAFPPG LWRQISPQPS TGLIYLIGHP EGQIKKIDGC TVIPLNERLK
601KYPNDCQDGL VDLYDTTSNV YCMFTQRSFL SEVWNTHTLS YDTCFSDGSS GSPVFNASGK
661LVALHTFGLF YQRGFNVHAL IEFGYSMDSI LCDIKKTNES LYKSLNDEKL ETYDEEKGKQ
721ESSLQDHQIE PMEC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

734 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.