Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM110B / C8orf72 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102329-100
Catalog Number: LS-G102329
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C8orf72(FAM110B) (NM_147189), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C8orf72 | FAM110B | Protein FAM110B
- Origin Species
- Human
- NCBI GenBank
- U79298
- SwissProt
- Q8TC76
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPTETLQTGS MVKPVSPAGT FTSAVPLRIL NKGPDYFRRQ AEPNPKRLSA VERLEADKAK61YVKSQEVINA KQEPVKPAVL AKPPVCPAAK RALGSPTLKV FGNHAKTESG VQRENLKLEI121LKNIINSSEG SSSGSGHKHS SRNWPPHRSE ATDLHRHSFA ESLKVYPTQG RRSPQEGGSH181VGRRLLEQSA ESFLHVSHSS SDIRKVTSVK PLKAIPCSSS APPLPPKPKI AAIASMKSPE241ADPVEPACGV SRRPSLQRSK SDLSDRYFRV DADVERFFNY CGLDPEELEN LGMENFARAN301SDIISLNFRS ASMISSDCEQ SQDSNSDLRN DDSANDRVPY GISAIERNAR IIKWLYSIKQ361ARESQKVSHVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
370 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.