Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAK / Focal Adhesion Kinase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115849-100
Catalog Number: LS-G115849
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAK(PTK2) (NM_005607), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FADK | Fadk1 | Focal adhesion kinase | FRNK | FADK 1 | Focal adhesion kinase 1 | FAK1 | p125FAK | PPP1R71 | Protein-tyrosine kinase 2 | PTK2 protein tyrosine kinase 2 | FAK | pp125 FA kinase | Pp125FAK | PTK2
- Origin Species
- Human
- NCBI GenBank
- NM_005607
- SwissProt
- Q05397
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 121.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MISADCNLCL PEYDRYLASS KIMAAAYLDP NLNHTPNSST KTHLGTGMER SPGAMERVLK61VFHYFESNSE PTTWASIIRH GDATDVRGII QKIVDSHKVK HVACYGFRLS HLRSEEVHWL121HVDMGVSSVR EKYELAHPPE EWKYELRIRY LPKGFLNQFT EDKPTLNFFY QQVKSDYMLE181IADQVDQEIA LKLGCLEIRR SYWEMRGNAL EKKSNYEVLE KDVGLKRFFP KSLLDSVKAK241TLRKLIQQTF RQFANLNREE SILKFFEILS PVYRFDKECF KCALGSSWII SVELAIGPEE301GISYLTDKGC NPTHLADFTQ VQTIQYSNSE DKDRKGMLQL KIAGAPEPLT VTAPSLTIAE361NMADLIDGYC RLVNGTSQSF IIRPQKEGER ALPSIPKLAN SEKQGMRTHA VSVSETDDYA421EIIDEEDTYT MPSTRDYEIQ RERIELGRCI GEGQFGDVHQ GIYMSPENPA LAVAIKTCKN481CTSDSVREKF LQEALTMRQF DHPHIVKLIG VITENPVWII MELCTLGELR SFLQVRKYSL541DLASLILYAY QLSTALAYLE SKRFVHRDIA ARNVLVSSND CVKLGDFGLS RYMEDSTYYK601ASKGKLPIKW MAPESINFRR FTSASDVWMF GVCMWEILMH GVKPFQGVKN NDVIGRIENG661ERLPMPPNCP PTLYSLMTKC WAYDPSRRPR FTELKAQLST ILEEEKAQQE ERMRMESRRQ721ATVSWDSGGS DEAPPKPSRP GYPSPRSSEG FYPSPQHMVQ TNHYQVSGYP GSHGITAMAG781SIYPGQASLL DQTDSWNHRP QEIAMWQPNV EDSTVLDLRG IGQVLPTHLM EERLIRQQQE841MEEDQRWLEK EERFLKPDVR LSRGSIDRED GSLQGPIGNQ HIYQPVGKPD PAAPPKKPPR901PGAPGHLGSL ASLSSPADSY NEGVKLQPQE ISPPPTANLD RSNDKVYENV TGLVKAVIEM961SSKIQPAPPE EYVPMVKEVG LALRTLLATV DETIPLLPAS THREIEMAQK LLNSDLGELI1021NKMKLAQQYV MTSLQQEYKK QMLTAAHALA VDAKNLLDVI DQARLKMLGQ TRPHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1074 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.