Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAF2 / ETEA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113831-100
Catalog Number: LS-G113831
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ETEA(FAF2) (NM_014613), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B17.2L | FAF2 | FAS-associated factor 2 | MMTN | KIAA0887 | UBX domain containing 8 | UBXN3B | Protein ETEA | ETEA | NDUNDUFA12L | UBX domain protein 3B | UBXD8
- Origin Species
- Human
- SwissProt
- Q96CS3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPEERDLT QEQTEKLLQF QDLTGIESMD QCRHTLEQHN WNIEAAVQDR LNEQEGVPSV61FNPPPSRPLQ VNTADHRIYS YVVSRPQPRG LLGWGYYLIM LPFRFTYYTI LDIFRFALRF121IRPDPRSRVT DPVGDIVSFM HSFEEKYGRA HPVFYQGTYS QALNDAKREL RFLLVYLHGD181DHQDSDEFCR NTLCAPEVIS LINTRMLFWA CSTNKPEGYR VSQALRENTY PFLAMIMLKD241RRMTVVGRLE GLIQPDDLIN QLTFIMDANQ TYLVSERLER EERNQTQVLR QQQDEAYLAS301LRADQEKERK KREERERKRR KEEEVQQQKL AEERRRQNLQ EEKERKLECL PPEPSPDDPE361SVKIIFKLPN DSRVERRFHF SQSLTVIHDF LFSLKESPEK FQIEANFPRR VLPCIPSEEW421PNPPTLQEAG LSHTEVLFVQ DLTDETRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.