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FAAH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAAH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105135-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAAH2 (NM_174912), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AMDD | FAAH2 | Fatty-acid amide hydrolase 2 | FAAH-2 | Amidase domain containing | Anandamide amidohydrolase 2 | RP11-479E16.1 | Fatty acid amide hydrolase 2 | Oleamide hydrolase 2
Origin Species
Human
NCBI GenBank
NM_174912
SwissProt
Q6GMR7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPSFTARIQ LFLLRALGFL IGLVGRAALV LGGPKFASKT PRPVTEPLLL LSGMQLAKLI
61RQRKVKCIDV VQAYINRIKD VNPMINGIVK YRFEEAMKEA HAVDQKLAEK QEDEATLENK
121WPFLGVPLTV KEAFQLQGMP NSSGLMNRRD AIAKTDATVV ALLKGAGAIP LGITNCSELC
181MWYESSNKIY GRSNNPYDLQ HIVGGSSGGE GCTLAAACSV IGVGSDIGGS IRMPAFFNGI
241FGHKPSPGVV PNKGQFPLAV GAQELFLCTG PMCRYAEDLA PMLKVMAGPG IKRLKLDTKV
301HLKDLKFYWM EHDGGSFLMS KVDQDLIMTQ KKVVVHLETI LGASVQHVKL KKMKYSFQLW
361IAMMSAKGHD GKEPVKFVDL LGDHGKHVSP LWELIKWCLG LSVYTIPSIG LALLEEKLRY
421SNEKYQKFKA VEESLRKELV DMLGDDGVFL YPSHPTVAPK HHVPLTRPFN FAYTGVFSAL
481GLPVTQCPLG LNAKGLPLGI QVVAGPFNDH LTLAVAQYLE KTFGGWVCPG KF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

532 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.