Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
F10 / Factor X Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99057-100
Catalog Number: LS-G99057
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Factor X(F10) (NM_000504), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Factor X | FX | F10 | Factor X deficiency | Stuart factor | Stuart-Prower factor | Coagulation factor X | Factor Xa | FXA | Prothrombinase
- NCBI GenBank
- NM_000504
- SwissProt
- P00742
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.54 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRPLHLVLL SASLAGLLLL GESLFIRREQ ANNILARVTR ANSFLEEMKK GHLERECMEE61TCSYEEAREV FEDSDKTNEF WNKYKDGDQC ETSPCQNQGK CKDGLGEYTC TCLEGFEGKN121CELFTRKLLD NGDCDQFCHE EQNSVVCSCA RGYTLADNGK ACIPTGPYPC GKQTLERRKR181SVAQATSSSG EAPDSITWKP YDAADLDPTE NPFDLLDFNQ TQPERGDNNL TRIVGGQECK241DGECPWQALL INEENEGFCG GTILSEFYIL TAAHCLYQAK RFKVRVGDRN TEQEEGGEAV301HEVEVVIKHN RFTKETYDFD IAVLRLKTPI TFRMNVAPAC LPERDWAEST LMTQKTGIVS361GFGRTHEKGR QSTRLKMLEV PYVDRNSCKL SSSFIITQNM FCAGYDTKQE DACQGDSGGP421HVTRFKDTYF VTGIVSWGEG CARKGKYGIY TKVTAFLKWI DRSMKTRGLP KAKSHAPEVI481TSSPLKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
486 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.