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EYA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EYA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105549-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EYA1 (NM_172059), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BOP | BOS1 | EYA1 | Eyes absent homolog 1 | BOR
Origin Species
Human
NCBI GenBank
NM_000503
SwissProt
Q99502

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEMQDLTSPH SRLSGSSESP SGPKLGNSHI NSNSMTPNGT EVKTEPMSSS ETASTTADGS
61LNNFSGSAIG SSSFSPRPTH QFSPPQIYPS NRPYPHILPT PSSQTMAAYG QTQFTTGMQQ
121ATAYATYPQP GQPYGISSYG IKTEGGLSQS QSPGQTGFLS YGTSFSTPQP GQAPYSYQMQ
181GSSFTTSSGI YTGNNSLTNS SGFNSSQQDY PSYPSFGQGQ YAQYYNSSPY PAHYMTSSNT
241SPTTPSTNAT YQLQEPPSGI TSQAVTDPTA EYSTIHSPST PIKDSDSDRL RRGSDGKSRG
301RGRRNNNPSP PPDSDLERVF IWDLDETIIV FHSLLTGSYA NRYGRECDQV HIDDVSSDDN
361GQDLSTYNFG TDGFPAAATS ANLCLATGVR GGVDWMRKLA FRYRRVKEIY NTYKNNVGGL
421LGPAKREAWL QLRAEIEALT DSWLTLALKA LSLIHSRTNC VNILVTTTQL IPALAKVLLY
481GLGIVFPIEN IYSATKIGKE SCFERIIQRF GRKVVYVVIG DGVEEEQGAK KHAMPFWRIS
541SHSDLMALHH ALELEYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

557 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.