Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EXOG / ENDOGL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116184-100
Catalog Number: LS-G116184
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENDOGL1(EXOG) (NM_005107), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Endonuclease G-like 1 | Endonuclease G-like 2 | ENGL | ENGL-b | ENGLA | ENGLB | ENDOGL1 | ENGL-a | EXOG | Nuclease EXOG | mitochondrial | Endo G-like 1 | ENDOGL2
- Origin Species
- Human
- NCBI GenBank
- NM_005107
- SwissProt
- Q9Y2C4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.08 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAIKSIASRL RGSRRFLSGF VAGAVVGAAG AGLAALQFFR SQGAEGALTG KQPDGSAEKA61VLEQFGFPLT GTEARCYTNH ALSYDQAKRV PRWVLEHISK SKIMGDADRK HCKFKPDPNI121PPTFSAFNED YVGSGWSRGH MAPAGNNKFS SKAMAETFYL SNIVPQDFDN NSGYWNRIEM181YCRELTERFE DVWVVSGPLT LPQTRGDGKK IVSYQVIGED NVAVPSHLYK VILARRSSVS241TEPLALGAFV VPNEAIGFQP QLTEFQVSLQ DLEKLSGLVF FPHLDRTSDI RNICSVDTCK301LLDFQEFTLY LSTRKIEGAR SVLRLEKIME NLKNAEIEPD DYFMSRYEKK LEELKAKEQS361GTQIRKPSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
368 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.