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EXOC6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EXOC6B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EXOC6B antibodies →
$725.00each
Catalog Number: LS-G113356-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXOC6B (NM_015189), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Exocyst complex component 6B | EXOC6B | KIAA0919 | SEC15-like 2 | SEC15-like 2 (S. cerevisiae) | SEC15-like protein 2 | SEC15B | WUGSC:H_NH0343N14.1 | SEC15 homolog B | SEC15L2
Origin Species
Human
NCBI GenBank
AK023791
SwissProt
Q9Y2D4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERGKMAEAE SLETAAEHER ILREIESTDT ACIGPTLRSV YDGEEHGRFM EKLETRIRNH
61DREIEKMCNF HYQGFVDSIT ELLKVRGEAQ KLKNQVTDTN RKLQHEGKEL VIAMEELKQC
121RLQQRNISAT VDKLMLCLPV LEMYSKLRDQ MKTKRHYPAL KTLEHLEHTY LPQVSHYRFC
181KVMVDNIPKL REEIKDVSMS DLKDFLESIR KHSDKIGETA MKQAQQQRNL DNIVLQQPRI
241GSKRKSKKDA YIIFDTEIES TSPKSEQDSG ILDVEDEEDD EEVPGAQDLV DFSPVYRCLH
301IYSVLGARET FENYYRKQRR KQARLVLQPP SNMHETLDGY RKYFNQIVGF FVVEDHILHT
361TQGLVNRAYI DELWEMALSK TIAALRTHSS YCSDPNLVLD LKNLIVLFAD TLQVYGFPVN
421QLFDMLLEIR DQYSETLLKK WAGIFRNILD SDNYSPIPVT SEEMYKKVVG QFPFQDIELE
481KQPFPKKFPF SEFVPKVYNQ IKEFIYACLK FSEDLHLSST EVDDMIRKST NLLLTRTLSN
541SLQNVIKRKN IGLTELVQII INTTHLEKSC KYLEEFITNI TNVLPETVHT TKLYGTTTFK
601DARHAAEEEI YTNLNQKIDQ FLQLADYDWM TGDLGNKASD YLVDLIAFLR STFAVFTHLP
661GKVAQTACMS ACKHLATSLM QLLLEAEVRQ LTLGALQQFN LDVRECEQFA RSGPVPGFQE
721DTLQLAFIDL RQLLDLFIQW DWSTYLADYG QPNCKYLRVN PVTALTLLEK MKDTSRKNNM
781FAQFRKNERD KQKLIDTVAK QLRGLISSHH S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

811 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.