Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EXOC6 / SEC15 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111473-100
Catalog Number: LS-G111473
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXOC6 (NM_019053), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EXOC6A | Exocyst complex component 6 | EXOC6 | SEC15 | SEC15A | SEC15L1 | Sec15p | SEC15-like protein 1 | SEC15L3 | SEC15-like 1 | SEC15-like 1 (S. cerevisiae) | SEC15-like protein 3 | SEC15L
- Origin Species
- Human
- NCBI GenBank
- AL137438
- SwissProt
- Q8TAG9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAENSESLGT VPEHERILQE IESTDTACVG PTLRSVYDDQ PNAHKKFMEK LDACIRNHDK61EIEKMCNFHH QGFVDAITEL LKVRTDAEKL KVQVTDTNRR FQDAGKEVIV HTEDIIRCRI121QQRNITTVVE KLQLCLPVLE MYSKLKEQMS AKRYYSALKT MEQLENVYFP WVSQYRFCQL181MIENLPKLRE DIKEISMSDL KDFLESIRKH SDKIGETAMK QAQHQKTFSV SLQKQNKMKF241GKNMYINRDR IPEERNETVL KHSLEEEDEN EEEILTVQDL VDFSPVYRCL HIYSVLGDEE301TFENYYRKQR KKQARLVLQP QSNMHETVDG YRRYFTQIVG FFVVEDHILH VTQGLVTRAY361TDELWNMALS KIIAVLRAHS SYCTDPDLVL ELKNLIVIFA DTLQGYGFPV NRLFDLLFEI421RDQYNETLLK KWAGVFRDIF EEDNYSPIPV VNEEEYKIVI SKFPFQDPDL EKQSFPKKFP481MSQSVPHIYI QVKEFIYASL KFSESLHRSS TEIDDMLRKS TNLLLTRTLS SCLLNLIRKP541HIGLTELVQI IINTTHLEQA CKYLEDFITN ITNISQETVH TTRLYGLSTF KDARHAAEGE601IYTKLNQKID EFVQLADYDW TMSEPDGRAS GYLMDLINFL RSIFQVFTHL PGKVAQTACM661SACQHLSTSL MQMLLDSELK QISMGAVQQF NLDVIQCELF ASSEPVPGFQ GDTLQLAFID721LRQLLDLFMV WDWSTYLADY GQPASKYLRV NPNTALTLLE KMKDTSKKNN IFAQFGKNDR781DKQKLIETVV KQLRSLVNGM SQHMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
804 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.