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EXOC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EXOC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EXOC5 antibodies →
$504.00each
Catalog Number: LS-G115239-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXOC5 (NM_006544), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EXOC5 | HSEC10 | SEC10L1 | SEC10P | SEC10-like 1 | PRO1912 | SEC10 | SEC10 (S. cerevisiae)-like 1 | SEC10-like 1 (S. cerevisiae) | Exocyst complex component 5
Origin Species
Human
NCBI GenBank
NM_006544
SwissProt
O00471

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATTAELFEE PFVADEYIER LVWRTPGGGS RGGPEAFDPK RLLEEFVNHI QELQIMDERI
61QRKVEKLEQQ CQKEAKEFAK KVQELQKSNQ VAFQHFQELD EHISYVATKV CHLGDQLEGV
121NTPRQRAVEA QKLMKYFNEF LDGELKSDVF TNSEKIKEAA DIIQKLHLIA QELPFDRFSE
181VKSKIASKYH DLECQLIQEF TSAQRRGEIS RMREVAAVLL HFKGYSHCVD VYIKQCQEGA
241YLRNDIFEDA GILCQRVNKQ VGDIFSNPET VLAKLIQNVF EIKLQSFVKE QLEECRKSDA
301EQYLKNLYDL YTRTTNLSSK LMEFNLGTDK QTFLSKLIKS IFISYLENYI EVETGYLKSR
361SAMILQRYYD SKNHQKRSIG TGGIQDLKER IRQRTNLPLG PSIDTHGETF LSQEVVVNLL
421QETKQAFERC HRLSDPSDLP RNAFRIFTIL VEFLCIEHID YALETGLAGI PSSDSRNANL
481YFLDVVQQAN TIFHLFDKQF NDHLMPLISS SPKLSECLQK KKEIIEQMEM KLDTGIDRTL
541NCMIRQMKHI LAAEQKKTDF KPEDENNVLI QYTNACVKVC AYVRKQVEKI KNSMDGKNVD
601TVLMELGVRF HRLIYEHLQQ YSYSCMGGML AICDVAEYRK CAKDFKIPMV LHLFDTLHAL
661CNLLVVAPDN LKQVCSGEQL ANLDKNILHS FVQLRADYRS ARLARHFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

708 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.