Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EXOC4 / SEC8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120695-100
Catalog Number: LS-G120695
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sec8(EXOC4) (NM_001037126), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Exocyst complex component Sec8 | Exocyst complex component 4 | KIAA1699 | Sec8p | SEC8-like 1 (S. cerevisiae) | SEC8 | EXOC4 | SEC8-like 1 | SEC8L1
- Origin Species
- Human
- NCBI GenBank
- NM_021807
- SwissProt
- Q96A65
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAEAAGGKY RSTVSKSKDP SGLLISVIRT LSTSDDVEDR ENEKGRLEEA YEKCDRDLDE61LIVQHYTELT TAIRTYQSIT ERITNSRNKI KQVKENLLSC KMLLHCKRDE LRKLWIEGIE121HKHVLNLLDE IENIKQVPQK LEQCMASKHY LSATDMLVSA VESLEGPLLQ VEGLSDLRLE181LHSKKMNLHL VLIDELHRHL YIKSTSRVVQ RNKEKGKISS LVKDASVPLI DVTNLPTPRK241FLDTSHYSTA GSSSVREINL QDIKEDLELD PEENSTLFMG ILIKGLAKLK KIPETVKAII301ERLEQELKQI VKRSTTQVAD SGYQRGENVT VENQPRLLLE LLELLFDKFN AVAAAHSVVL361GYLQDTVVTP LTQQEDIKLY DMADVWVKIQ DVLQMLLTEY LDMKNTRTAS EPSAQLSYAS421TGREFAAFFA KKKPQRPKNS LFKFESSSHA ISMSAYLREQ RRELYSRSGE LQGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
473 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.