Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EXOC3L4 / C14orf73 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120033-100
Catalog Number: LS-G120033
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXOC3L4 (NM_001077594), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001077594
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSPQTDTPG PELQSPKEAE EPQTPAQGSR RTSSRKEPNA HRKDGTRLGL GSLRQAFSRA61SQRALTQVSK EDTGLFRRSS CSLFRSFRQA LNDGPATGHS QATPEVPSGV MNGVSQQAST121GAASEELKPE AEGKSVADLI TERQLLAAFE QLLRLETLLV AEKASRTFEQ DPTAFARRAM181DVCLLYDGLA AEIGAIVRET LDSDGVDAAA LAELARVVSA EEEAHPSPPD DGDFLRTPRR241WRQHWEEAVR RSAQERVRRP GAGWAFGEAE GASGLAQLLA ELGGLVRRDL QKVRQEVQPA301YAAAGFPAWE VYLRAFHSAV AQRLQELARD ARGCEQLYIL LDWAANVYGS PDFLGAPGLA361LPAEPLPPLL APDVWARLES DYTSFLEAKI ASCFDSILQL EQSHWAAAEV PEVLQGLYQA421PLSMDVHMLV AEHVKAAGAI SAELEATTLR ICTRALGLFV PRFEKAFLAS EAVSEPHLGA481YINACEELRT SLLSRFPGTQ EELEKPLVTA TCSFQKHLLQ GLQRELQPLF RVVCTRDWLT541QDWLHPLMDK VVTFAGHLQR VARPRAQETL QEVHRFVVRE YLARALRPRE RFRGMERMHG601SQKMSLDAQA ISDTFQGLGS EATWLDQAIQ CVAEILGETY KDDIQRHLET LIRSYPDIRR661DHILAILALR RLGRQRNQHL LQHTQDLLRA AAGAAGAEAP RGRVLFEEIK VPSAMAVLIT721CVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
722 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.