Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EXOC3 / SEC6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101011-100
Catalog Number: LS-G101011
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXOC3 (NM_007277), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Exocyst complex component 3 | Exocyst complex component Sec6 | EXOC3 | SEC6L1 | Sec6p | Sec 6 homolog | SEC6-like 1 (S. cerevisiae) | SEC6 | SEC6-like 1
- Origin Species
- Human
- NCBI GenBank
- NM_001024964
- SwissProt
- Q62825
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKETDREAVA TAVQRVAGML QRPDQLDKVE QYRRREARKK ASVEARLKAA IQSQLDGVRT61GLSQLHNALN DVKDIQQSLA DVSKDWRQSI NTIESLKDVK DAVVQHSQLA AAVENLKNIF121SVPEIVRETQ DLIEQGALLQ AHRKLMDLEC SRDGLMYEQY RMDSGNTRDM TLIHGYFGST181QGLSDELAKQ LWMVLQRSLV TVRRDPTLLV SVVRIIEREE KIDRRILDRK KQTGFVPPGR241PKNWKEKMFT ILERTVTTRI EGTQADTRES DKMWLVRHLE IIRKYVLDDL IVAKNLMVQC301FPPHYEIFKN LLNMYHQALS TRMQDLASED LEANEIVSLL TWVLNTYTST EMMRNVELAP361EVDVGTLEPL LSPHVVSELL DTYMSTLTSN IIAWLRKALE TDKKDWVKET EPEADQDGYY421QTTLPAIVFQ MFEQNLQVAA QISEDLKTKV LVLCLQQMNS FLSRYKDEAQ LYKEEHLRNR481QHPHCYVQYM IAIINNCQTF KESIVSLKRK YLKNEVEEGV SPSQPSMDGI LDAIAKEGCS541GLLEEVFLDL EQHLNELMTK KWLLGSNAVD IICVTVEDYF NDFAKIKKPY KKRMTAEAHR601RVVVEYLRAV MQKRISFRSP EERKEGAEKM VREAEQLRFL FRKLASGFGE DVDGYCDTIV661AVAEVIKLTD PSLLYLEVST LVSKYPDIRD DHIGALLAVR GDASRDMKQT IMETLEQGPA721QASPSYVPLF KDIVVPSLNV AKLLKSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
745 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.