Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EXOC1 / SEC3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104731-100
Catalog Number: LS-G104731
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXOC1 (NM_178237), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BM-102 | Exocyst complex component 1 | EXOC1 | SEC3-like 1 (S. cerevisiae) | SEC3L1 | SEC3P | SEC3-like 1 | Exocyst complex component Sec3 | SEC3
- Origin Species
- Human
- NCBI GenBank
- NM_018261
- SwissProt
- Q9NV70
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAIKHALQR DIFTPNDERL LSIVNVCKAG KKKKNCFLCA TVTTERPVQV KVVKVKKSDK61GDFYKRQIAW ALRDLAVVDA KDAIKENPEF DLHFEKIYKW VASSTAEKNA FISCIWKLNQ121RYLRKKIDFV NVSSQLLEES VPSGENQSVT GGDEEVVDEY QELNAREEQD IEIMMEGCEY181AISNAEAFAE KLSRELQVLD GANIQSIMAS EKQVNILMKL LDEALKEVDQ IELKLSSYEE241MLQSVKEQMD QISESNHLIH LSNTNNVKLL SEIEFLVNHM DLAKGHIKAL QEGDLASSRG301IEACTNAADA LLQCMNVALR PGHDLLLAVK QQQQRFSDLR ELFARRLASH LNNVFVQQGH361DQSSTLAQHS VELTLPNHHP FHRDLLRYAK LMEWLKSTDY GKYEGLTKNY MDYLSRLYER421EIKDFFEVAK IKMTGTTKES KKFGLHGSSG KLTGSTSSLN KLSVQSSGNR RSQSSSLLDM481GNMSASDLDV ADRTKFDKIF EQVLSELEPL CLAEQDFISK FFKLQQHQSM PGTMAEAEDL541DGGTLSRQHN CGTPLPVSSE KDMIRQMMIK IFRCIEPELN NLIALGDKID SFNSLYMLVK601MSHHVWTAQN VDPASFLSTT LGNVLVTVKR NFDKCISNQI RQMEEVKISK KSKVGILPFV661AEFEEFAGLA ESIFKNAERR GDLDKAYTKL IRGVFVNVEK VANESQKTPR DVVMMENFHH721IFATLSRLKI SCLEAEKKEA KQKYTDHLQS YVIYSLGQPL EKLNHFFEGV EARVAQGIRE781EEVSYQLAFN KQELRKVIKE YPGKEVKKGL DNLYKKVDKH LCEEENLLQV VWHSMQDEFI841RQYKHFEGLI ARCYPGSGVT MEFTIQDILD YCSSIAQSHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
879 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.