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EXD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EXD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EXD1 antibodies →
$504.00each
Catalog Number: LS-G106128-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXDL1(EXD1) (NM_152596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EXD1 | EXDL1 | Exonuclease 3-5 domain-like 1
Origin Species
Human
NCBI GenBank
NM_001286441
SwissProt
Q8NHP7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDSEFLAYV ELLDEVEQGS VRAKASSVSL HAERTWMEKM KVEDLNVCEP ASPAPEAPAT
61SLLNDLKYSP SEEEEVTYTV INQFQQKFGA AILHIKKQNV LSVAAEGANV CRHGKLCWLQ
121VATNCRVYLF DIFLLGSRAF HNGLQMILED KRILKVIHDC RWLSDCLSHQ YGILLNNVFD
181TQVADVLQFS METGGYLPNC ITTLQESLIK HLQVAPKYLS FLEKRQKLIQ ENPEVWFIRP
241VSPSLLKILA LEATYLLPLR LALLDEMMSD LTTLVDGYLN TYREGSADRL GGTEPTCMEL
301PEELLQLKDF QKQRREKAAR EYRVNAQGLL IRTVLQPKKL VTETAGKEEK VKGFLFGKNF
361RIDKAPSFTS QDFHGDVNLL KEESLNKQAT NPQHLPPTEE GETSEDSSNK LICTKSKGSE
421DQRITQKEHF MTPKHEFQAS LSLKEETEQL LMVENKEDLK CTKQAVSMSS FPQETRVSPS
481DTFYPIRKTV VSTLPPCPAL EKIDSWISPF LNLP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.