EWSR1 / EWS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129082-100
Catalog Number: LS-G129082
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EWSR1 (NM_013986), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EWS | RNA-binding protein EWS | BK984G1.4 | EWS oncogene | EWSR1
- Origin Species
- Human
- NCBI GenBank
- NM_005243
- SwissProt
- Q01844
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASTDYSTYS QAAAQQGYSA YTAQPTQGYA QTTQAYGQQS YGTYGQPTDV SYTQAQTTAT61YGQTAYATSY GQPPTVEGTS TGYTTPTAPQ AYSQPVQGYG TGAYDTTTAT VTTTQASYAA121QSAYGTQPAY PAYGQQPAAT APTRPQDGNK PTETSQPQSS TGGYNQPSLG YGQSNYSYPQ181VPGSYPMQPV TAPPSYPPTS YSSTQPTSYD QSSYSQQNTY GQPSSYGQQS SYGQQSSYGQ241QPPTSYPPQT GSYSQAPSQY SQQSSSYGQQ SSFRQDHPSS MGVYGQESGG FSGPGENRSM301SGPDNRGRGR GGFDRGGMSR GGRGGGRGGM GAGERGGFNK PGGPMDEGPD LDLGPPVDPD361EDSDNSAIYV QGLNDSVTLD DLADFFKQCG VVKMNKRTGQ PMIHIYLDKE TGKPKGDATV421SYEDPPTAKA AVEWFDGKDF QGSKLKVSLA RKKPPMNSMR GGLPPREGRG MPPPLRGGPG481GPGGPGGPMG RMGGRGGDRG GFPPRGPRGS RGNPSGGGNV QHRAGDWQCP NPGCGNQNFA541WRTECNQCKA PKPEGFLPPP FPPPGGDRGR GGPGGMRGGR GGLMDRGGPG GMFRGGRGGD601RGGFRGGRGM DRGGFGGGRR GGPGGPPGPL MEQMGGRRGG RGGPGKMDKG EHRQERRDRP661YTRTRPLEQKLISEEDLAANDILDYKDDDDKV
661 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.