Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EWSR1 / EWS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100494-100
Catalog Number: LS-G100494
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EWSR1 (NM_005243), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EWS | RNA-binding protein EWS | BK984G1.4 | EWS oncogene | EWSR1
- Origin Species
- Human
- NCBI GenBank
- NM_005243
- SwissProt
- Q01844
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASTDYSTYS QAAAQQGYSA YTAQPTQGYA QTTQAYGQQS YGTYGQPTDV SYTQAQTTAT61YGQTAYATSY GQPPTGYTTP TAPQAYSQPV QGYGTGAYDT TTATVTTTQA SYAAQSAYGT121QPAYPAYGQQ PAATAPTRPQ DGNKPTETSQ PQSSTGGYNQ PSLGYGQSNY SYPQVPGSYP181MQPVTAPPSY PPTSYSSTQP TSYDQSSYSQ QNTYGQPSSY GQQSSYGQQS SYGQQPPTSY241PPQTGSYSQA PSQYSQQSSS YGQQSSFRQD HPSSMGVYGQ ESGGFSGPGE NRSMSGPDNR301GRGRGGFDRG GMSRGGRGGG RGGMGAGERG GFNKPGGPMD EGPDLDLGPP VDPDEDSDNS361AIYVQGLNDS VTLDDLADFF KQCGVVKMNK RTGQPMIHIY LDKETGKPKG DATVSYEDPP421TAKAAVEWFD GKDFQGSKLK VSLARKKPPM NSMRGGLPPR EGRGMPPPLR GGPGGPGGPG481GPMGRMGGRG GDRGGFPPRG PRGSRGNPSG GGNVQHRAGD WQCPNPGCGN QNFAWRTECN541QCKAPKPEGF LPPPFPPPGG DRGRGGPGGM RGGRGGLMDR GGPGGMFRGG RGGDRGGFRG601GRGMDRGGFG GGRRGGPGGP PGPLMEQMGG RRGGRGGPGK MDKGEHRQER RDRPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
655 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.