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EVI5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EVI5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EVI5 antibodies →
$725.00each
Catalog Number: LS-G100611-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EVI5 (NM_005665), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EVI5 | EVI-5 | Evi5 homolog | NB4S
Origin Species
Human
NCBI GenBank
AF008915
SwissProt
O60447

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVTNKMTAAF RNPSGKQVAT DKVAEKLSST LSWVKNTVSH TVSQMASQVA SPSTSLHTTS
61SSTTLSTPAL SPSSPSQLSP DDLELLAKLE EQNRLLETDS KSLRSVNGSR RNSGSSLVSS
121SSASSNLSHL EEDSWILWGR IVNEWEDVRK KKEKQVKELV HKGIPHHFRA IVWQLLCSAQ
181SMPIKDQYSE LLKMTSPCEK LIRRDIARTY PEHNFFKEKD SLGQEVLFNV MKAYSLVDRE
241VGYCQGSAFI VGLLLMQMPE EEAFCVFVKL MQDYRLRELF KPSMAELGLC MYQFECMIQE
301HLPELFVHFQ SQSFHTSMYA SSWFLTIFLT TFPLPVATRI FDIFMSEGLE IVFRVGLALL
361QMNQAELMQL DMEGMLQHFQ KVIPHQFDGV PDKLIQAAYQ VKYNSKKMKK LEKEYTTIKT
421KEMEEQVEIK RLRTENRLLK QRIETLEKHK CSSNYNEDFV LQLEKELVQA RLSEAESQCA
481LKEMQDKVLD IEKRNNSLPD ENNIARLQEE LIAVKLREAE AIMGLKELRQ QVKDLEEHWQ
541RHLARTTGRW KDPPKKNAMN ELQDELMTIR LREAETQAEI REIKQRMMEM ETQNQINSNH
601LRRAEQEVIS LQEKVQYLSA QNKGLLTQLS EAKRKQAEIE CKNKEEVMAV RLREADSIAA
661VAELRQHIAE LEIQKEEGKL QGQLNKSDSN QYIGELKDQI AELNHELRCL KGQRGFSGQP
721PFDGIHIVNH LIGDDESFHS SDEDFIDNSL QETGVGFPLH GKSGSMSLDP AVADGSESET
781EDSVLETRES NQVVQKERPP RRRESYSTTV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

810 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.