Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ETV5 / ERM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100300-100
Catalog Number: LS-G100300
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ETV5(ETV5) (NM_004454), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ETS translocation variant 5 | ERM | Ets variant 5 | Ets-related molecule | Ets-related protein ERM | ETV5
- Origin Species
- Human
- NCBI GenBank
- NM_004454
- SwissProt
- P41161
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDGFYDQQVP FMVPGKSRSE ECRGRPVIDR KRKFLDTDLA HDSEELFQDL SQLQEAWLAE61AQVPDDEQFV PDFQSDNLVL HAPPPTKIKR ELHSPSSELS SCSHEQALGA NYGEKCLYNY121CAYDRKPPSG FKPLTPPTTP LSPTHQNPLF PPPQATLPTS GHAPAAGPVQ GVGPAPAPHS181LPEPGPQQQT FAVPRPPHQP LQMPKMMPEN QYPSEQRFQR QLSEPCHPFP PQPGVPGDNR241PSYHRQMSEP IVPAAPPPPQ GFKQEYHDPL YEHGVPGMPG PPAHGFQSPM GIKQEPRDYC301VDSEVPNCQS SYMRGGYFSS SHEGFSYEKD PRLYFDDTCV VPERLEGKVK QEPTMYREGP361PYQRRGSLQL WQFLVTLLDD PANAHFIAWT GRGMEFKLIE PEEVARRWGI QKNRPAMNYD421KLSRSLRYYY EKGIMQKVAG ERYVYKFVCD PDALFSMAFP DNQRPFLKAE SECHLSEEDT481LPLTHFEDSP AYLLDMDRCS SLPYAEGFAYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
510 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.