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ETV3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ETV3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126642-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ETV3 (NM_001145312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA110J1.4 | ETS translocation variant 3 | ETV3 | Ets variant gene 3 | Ets variant 3 | PE-1 | PE1 | METS
Origin Species
Human
NCBI GenBank
L16464
SwissProt
P41162

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKAGCSIVEK PEGGGGYQFP DWAYKTESSP GSRQIQLWHF ILELLQKEEF RHVIAWQQGE
61YGEFVIKDPD EVARLWGRRK CKPQMNYDKL SRALRYYYNK RILHKTKGKR FTYKFNFNKL
121VMPNYPFINI RSSGVVPQSA PPVPTASSRF HFPPLDTHSP TNDVQPGRFS ASSLTASGQE
181SSNGTDRKTE LSELEDGSAA DWRRGVDPVS SRNAIGGGGI GHQKRKPDIM LPLFARPGMY
241PDPHSPFAVS PIPGRGGVLN VPISPALSLT PTIFSYSPSP GLSPFTSSSC FSFNPEEMKH
301YLHSQACSVF NYHLSPRTFP RYPGLMVPPL QCQMHPEEST QFSIKLQPPP VGRKNRERVE
361SSEESAPVTT PTMASIPPRI KVEPASEKDP ESLRQSAREK EEHTQEEGTV PSRTIEEEKG
421TIFARPAAPP IWPSVPISTP SGEPLEVTED SEDRPGKEPS APEKKEDALM PPKLRLKRRW
481NDDPEARELS KSGKFLWNGS GPQGLATAAA DA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

512 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.