Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ETS1 / ETS-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126200-100
Catalog Number: LS-G126200
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ETS1 (NM_001143820), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ets protein | ETS-1 | EWSR2 | p54 | ETS1 | Protein C-ets-1
- Origin Species
- Human
- NCBI GenBank
- NM_005238
- SwissProt
- P14921
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSYFVDSAGS SPVPYSAPRP AVVRQGPSNT YEDPRMNCGF QSNYHQQRPC YPFWDEMATQ61EVPTGLEHCV SDMECADVPL LTPSSKEMMS QALKATFSGF TKEQQRLGIP KDPRQWTETH121VRDWVMWAVN EFSLKGVDFQ KFCMNGAALC ALGKDCFLEL APDFVGDILW EHLEILQKED181VKPYQVNGVN PAYPESRYTS DYFISYGIEH AQCVPPSEFS EPSFITESYQ TLHPISSEEL241LSLKYENDYP SVILRDPLQT DTLQNDYFAI KQEVVTPDNM CMGRTSRGKL GGQDSFESIE301SYDSCDRLTQ SWSSQSSFNS LQRVPSYDSF DSEDYPAALP NHKPKGTFKD YVRDRADLNK361DKPVIPAAAL AGYTGSGPIQ LWQFLLELLT DKSCQSFISW TGDGWEFKLS DPDEVARRWG421KRKNKPKMNY EKLSRGLRYY YDKNIIHKTA GKRYVYRFVC DLQSLLGYTP EELHAMLDVK481PDADETRTRPLEQKLISEEDLAANDILDYKDDDDKV
485 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.