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ETAA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ETAA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ETAA1 antibodies →
$504.00each
Catalog Number: LS-G111520-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ETAA1 (NM_019002), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ETAA1 | ETAA16
Origin Species
Human
NCBI GenBank
NM_019002
SwissProt
Q9NY74

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRRRKHDDS PSPKKTPHKT VAAEECGSVV EPGRRRLRSA RGSWPCGARE GPPGPVRQRE
61QPPTAALCSK SNPEERYETP KRALKMDSLS SSFSSPNDPD GQNDIFWDQN SPLTKQLGKG
121RKKQIYTTDS DEISHIVNRI APQDEKPTTN SMLDMWIGET AIPCTPSVAK GKSRAKISCT
181KLKTQSQEEE LMKLAKQFDK NMEELDVIQE QNKRNYDFTQ MISETEILSN YKDNIQMWSL
241HNIVPEIDNA TKKPIKGNTK ISVANNQNSS QKPFDQIAEA AFNAIFDGST QKCSGQLSQE
301LPEAFWSTSN TTFVKTNALK EEKIITNETL VIEKLSNKTP RSLSSQVDTP IMTKSCVTSC
361TKEPETSNKY IDAFTTSDFE DDWENLLGSE PFAMQNIDMP ELFPSKTAHV TDQKEICTFN
421SKTVKNTSRA NTSPDARLGD SKVLQDLSSK TYDRELIDAE YRFSPNSNKS NKLSTGNKMK
481FENSSNKIVI QDEIQNCIVT SNLTKIKEDI LTNSTEASER KSALNTRYSN EQKNKCILNQ
541SIKAPVNTDL FGSANLGSKT SVSNPNQTSA SKVGSFFDDW NDPSFANEII KACHQLDNTW
601EADDVDDDLL YQACDDIERL TQQQDIRKDS KTSESICEIN NNSEHGAKLT QQQDIRKDSK
661TSESICEINN NSEHGAKNMF AISKQGSNLV QSKHLNPGSI SVQTSLTNSS QIDKPMKMEK
721GEMYGNSPRF LGATNLTMYS KISNCQINNL HVSYTNTDVP IQVNSSKLVL PGSSSLNVTS
781DHMNTEITTY KKKLSTNQPC HKTVTDEAQS NLNTTVGFSK FTFTRMKNSQ ILSQFNQNCI
841TGSMSDTKIT QGVEKKKGVN PLLEEAVGQQ SLVKLSESLK QSSKEEEEKN RKCSPEEIQR
901KRQEALVRRM AKARASSVNA APTSFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

926 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.