Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ESYT2 / E-Syt2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111038-100
Catalog Number: LS-G111038
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM62B(ESYT2) (NM_020728), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CHR2SYT | Chr2 synaptotagmin | ESYT2 | FAM62B | KIAA1228 | E-Syt2 | Extended synaptotagmin-2
- Origin Species
- Human
- NCBI GenBank
- AK001181
- SwissProt
- A0FGR8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTPPSRAEAG VRRSRVPSEG RWRGAEPPGI SASTQPASAG RAARHCGAMS GARGEGPEAG61AGGAGGRAAP ENPGGVLSVE LPGLLAQLAR SFALLLPVYA LGYLGLSFSW VLLALALLAW121CRRSRGLKAL RLCRALALLE DEERVVRLGV RACDLPAWVH FPDTERAEWL NKTVKHMWPF181ICQFIEKLFR ETIEPAVRGA NTHLSTFSFT KVDVGQQPLR INGVKVYTEN VDKRQIILDL241QISFVGNCEI DLEIKRYFCR AGVKSIQIHG TMRVILEPLI GDMPLVGALS IFFLRKPLLE301INWTGLTNLL DVPGLNGLSD TIILDIISNY LVLPNRITVP LVSEVQIAQL RFPVPKGVLR361IHFIEAQDLQ GKDTYLKGLV KGKSDPYGII RVGNQIFQSR VIKENLSPKW NEVYEALVYE421HPGQELEIEL FDEDPDKDDF LGSLMIDLIE VEKERLLDEW FTLDEVPKGK LHLRLEWLTL481MPNASNLDKV LTDIKADKDQ ANDGLSSALL ILYLDSARNL PSGKKISSNP NPVVQMSVGH541KAQESKIRYK TNEPVWEENF TFFIHNPKRQ DLEVEVRDEQ HQCPLGNLKV PLSQLLTSED601MTVSQRFQLG NSGPNSTIKM KIALRVLHLE KRERPPDHQH SAQVKRPSVS KEGRKTSIKS661HMSGSPGPGG SNTAPSTPVI GGSDKPGMEE KAQPPEAGPQ GLHDLGRSSS SLLASPGHIS721VKEPTPSIAS DISLPIATQE LRQRLRQLEN GTTLGQSPLG QIQLTIRHSS QRNKLIVVVH781ACRNLIAFSE DGSDPYVRMY LLPDKRRSGR RKTHVSKKTL NPVFDQSFDF SVSLPEVQRR841TLDVAVKNSG GFLSKDKGLL GKVLVALASE ELAKGWTQWY DLTEDGTRPQ AMTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
893 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.