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ESPNL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ESPNL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ESPNL antibodies →
$725.00each
Catalog Number: LS-G103927-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ESPNL (NM_194312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Espin-like | Espin-like protein | ESPNL
Origin Species
Human
NCBI GenBank
NM_194312
SwissProt
Q6ZVH7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKQRALVAA KDGDVATLER LLEAGALGPG ITDALGAGLV HHATRAGHLD CVKFLVQRAQ
61LPGNQRAHNG ATPAHDAAAT GSLAELCWLV REGGCGLQDQ DASGVSPLHL AARFGHPVLV
121EWLLHEGHSA TLETREGARP LHHAAVSGDL TCLKLLTAAH GSSVNRRTRS GASPLYLACQ
181EGHLHLAQFL VKDCGADVHL RALDGMSALH AAAARGHYSL VVWLVTFTDI GLTARDNEGA
241TALHFAARGG HTPILDRLLL MGTPILRDSW GGTPLHDAAE NGQMECCQTL VSHHVDPSLR
301DEDGYTAADL AEYHGHRDCA QYLREVAQPV PLLMTPPPPP FPPPPLLATR RSLEDGRRGG
361PGPGNPSPMS LSPAWPGHPD QPLPREQMTS PAPPRIITSA TADPEGTETA LAGDTSDGLA
421ALQLDGLPSG DIDGLVPTRD ERGQPIPEWK RQVMVRKLQA RLGAESSAEA QDNGGSSGPT
481EQAAWRYSQT HQAILGPFGE LLTEDDLVYL EKQIADLQLR RRCQEYESEL GRLAAELQAL
541LPEPLVSITV NSHFLPRAPG LEVEEASIPA AEPAGSAEAS EVAPGVQPLP FWCSHISRLV
601RSLSLLLKGV HGLVQGDEKP STRPLQDTCR EASASPPRSE AQRQIQEWGV SVRTLRGNFE
661SASGPLCGFN PGPCEPGAQH RQCLSGCWPA LPKPRSGLAS GEPRPGDTEE ASDSGISCEE
721VPSEAGAAAG PDLASLRKER IIMLFLSHWR RSAYTPALKT VACRTLGARH AGLRGQEAAR
781SPGPPSPPSE GPRLGHLWQQ RSTITHLLGN WKAIMAHVPA RQLRRLSRQP RGALSPEQFL
841PHVDGAPVPY SSLSLDLFML GYFQLLECDL PAEERKLRHL LCFEVFEHLG THGWEAVRAF
901HKAVTDEVAA GRRAWTDGFE DIKARFFGSS QRPAWDTEPG RKSGLTLLGP LPHAAVPCSG
961PEPTAQRLGS RSQQGSFNGE DICGYINRSF AFWKEKEAEM FNFGE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1005 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.