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ERVV-2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ERVV-2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ERVV-2 antibodies →
$504.00each
Catalog Number: LS-G131020-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ERVV (NM_001191055), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001191055

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTEKFLFLYL SLLPMPLLSQ AQWNENSLVS FSKIIASGNH LSNCWICHNF ITRSSSYQYI
61LVRNFSLNLT FGSGIPEGQH KSVPLQVSLA NSAHQVPCLD LTPPFNQSSK TSFYFYNCSS
121LNQTCCPCPE GHCDRKNTSE EGFPSPTIHP MSFSPAGCHP NLTHWCPAKQ MNDYRDKSPQ
181NRCAAWEGKE LITWRVLYSL PKAHTVPTWP KSTVPLGGPL SPACNQTIPA GWKSQLHKWF
241DSHIPRWACT PPGYVFLCGP QKNKLPFDGS PKITYSTPPV ANLYTCINNI QHTGECAVGL
301LGPRGIGVTI YNTTQPRQKR ALGLILAGMG AAIGMIAPWG GFTYHDVTLR NLSRQIDNIA
361KSTRDSISKL KASIDSLANV VMDNRLALDY LLAEQGGVCA VINKSCCVYV NNSGAIEEDI
421KKIYDEATWL HDFGKGGASA RAIWEAVKSA LPSLNWFVPL LGPATVILLL FLFGPCFFNL
481LIKCVSSRIK QFHMKSPQME RYQLSVIGGP STYKHISPLD ASGQRFRETM EEFSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

535 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.