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ERN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ERN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ERN2 antibodies →
$725.00each
Catalog Number: LS-G108335-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IRE1(ERN2) (NM_033266), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ER to nucleus signalling 2 | HIRE2p | Inositol-requiring protein 2 | IRE1-BETA | Inositol-requiring 1 beta | IRE1 beta | IRE2 | ERN2 | IRE1b
Origin Species
Human
NCBI GenBank
AB047079
SwissProt
Q76MJ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
107 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRAGIGEDS RLGLQAQPGA EPSPGRAGTE RSLGGAQGPG QPCSCPGAMA SAVRGSRPWP
61RLGLQLQFAA LLLGTLSPQV HTLRPENLLL VSTLDGSLHA LSKQTGDLKW TLRDDPVIEG
121PMYVTEMAFL SDPADGSLYI LGTQKQQGLM KLPFTIPELV HASPCRSSDG VFYTGRKQDA
181WFVVDPESGE TQMTLTTEGP STPRLYIGRT QYTVTMHDPR APALRWNTTY RRYSAPPMDG
241SPGKYMSHLA SCGMGLLLTV DPGSGTVLWT QDLGVPVMGV YTWHQDGLRQ LPHLTLARDT
301LHFLALRWGH IRLPASGPRD TATLFSTLDT QLLMTLYVGK DETGFYVSKA LVHTGVALMP
361RGLTLAPADG PTTDEVTLQV SGEREGSPST AVRYPSGSVA LPSQWLLIGH HELPPVLHTT
421MLRVHPTLGS GTAETRPPEN TQAPAFFLEL LSLSREKLWD SELHPEEKTP DSYLGLGPQD
481LLAASLTAVL LGGWILFVMR QQQPQVVEKQ QETPLAPADF AHISQDAQSL HSGASRRSQK
541RLQSPSKQAQ PLDDPEAEQL TVVGKISFNP KDVLGRGAGG TFVFRGQFEG RAVAVKRLLR
601ECFGLVRREV QLLQESDRHP NVLRYFCTER GPQFHYIALE LCRASLQEYV ENPDLDRGGL
661EPEVVLQQLM SGLAHLHSLH IVHRDLKPGN ILITGPDSQG LGRVVLSDFG LCKKLPAGRC
721SFSLHSGIPG TEGWMAPELL QLLPPDSPTS AVDIFSAGCV FYYVLSGGSH PFGDSLYRQA
781NILTGAPCLA HLEEEVHDKV VARDLVGAML SPLPQPRPSA PQVLAHPFFW SRAKQLQFFQ
841DVSDWLEKES EQEPLVRALE AGGCAVVRDN WHEHISMPLQ TDLRKFRSYK GTSVRDLLRA
901VRNKKHHYRE LPVEVRQALG QVPDGFVQYF TNRFPRLLLH THRAMRSCAS ESLFLPYYPP
961DSEARRPCPG ATGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

974 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.