Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERLIN1 / SPFH1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115306-100
Catalog Number: LS-G115306
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ERLIN1 (NM_006459), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C10orf69 | Erlin-1 | ERLIN1 | ER lipid raft associated 1 | KEO4 | KE04 | RP11-316M21.1 | SPFH domain family | member 1 | Protein KE04 | SPFH1
- Origin Species
- Human
- NCBI GenBank
- NM_006459
- SwissProt
- O75477
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTQARVLVAA VVGLVAVLLY ASIHKIEEGH LAVYYRGGAL LTSPSGPGYH IMLPFITTFR61SVQTTLQTDE VKNVPCGTSG GVMIYIDRIE VVNMLAPYAV FDIVRNYTAD YDKTLIFNKI121HHELNQFCSA HTLQEVYIEL FDQIDENLKQ ALQKDLNLMA PGLTIQAVRV TKPKIPEAIR181RNFELMEAEK TKLLIAAQKQ KVVEKEAETE RKKAVIEAEK IAQVAKIRFQ QKVMEKETEK241RISEIEDAAF LAREKAKADA EYYAAHKYAT SNKHKLTPEY LELKKYQAIA SNSKIYFGSN301IPNMFVDSSC ALKYSDIRTG RESSLPSKEA LEPSGENVIQ NKESTGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
346 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.