Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERICH5 / C8orf47 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105197-100
Catalog Number: LS-G105197
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C8orf47(ERICH5) (NM_173549), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001170806
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGCSSSALNK AGDSSRFPSV TSNEHFSTAE ESESCFAQPK PHALGRESTV DGNVQRESRP61PLQKLKVSAE PTANGVKPLQ EQPLAKDVAP GRDATDQSGS TEKTQPGEGL EESGPPQPGG121KEDAPAAEGK KKDAGAGTEA ESLKGNAEAQ PLGPEAKGQP LQAAVEKDSL RAVEVTENPQ181TAAEMKPLGT TENVLTLQIA GELQPQGTVG KDEQAPLLET ISKENESPEI LEGSQFVETA241EEQQLQATLG KEEQPQLLER IPKENVTPEV LDRSQLVEKP VMNDPFHKTP EGPGNMEQIQ301PEGIVGSMEH PARNVEAGAY VEMIRNIHTN EEDQRIEGET GEKVETDMEN EKVSEGAETK361EEETGEVVDL SAATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
374 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.