Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERICH3 / C1orf173 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122587-100
Catalog Number: LS-G122587
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf173(ERICH3) (NM_001002912), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001002912
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 168.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSHSHPAGLL AAYNSLMDKH LAGYFNNTRI RRHLLRSGLI TRSGRILSEK EYKLNMMKRD61HQKYIRECLA QAIFHKVLDM ERYHQLEIKK KLETLARKER IQRFKGEHTR RSVENNMPIL121SPHPPVGPKS NRGHSVLVDE GHSSPLALTA PRPYTAPGNM QPPIRLQPLP SNPAVETVPK181VTSRSRSKTS LLENEALFPI GGKKAVMKFR NSIGNSQRMN SYQLPNINSY MMPIPPPLPP241TGKITRENRS ETWRRRRFRP TTAPNGLEPL LTKDSRRIHK TSLHSNAAIT MIYLGKNVHL301SSDNPDFRDE IKVYQQHCGG ENLCVYKGKL LEKETFQFIS KRHHGFPFSL TFFLNGMQVN361RLSSCCEYKH RKGSRLGGKR GYFGFVCVER SSPCYKCIIA MGLDKKPSLP KSRKEKSTEK421GEELKKAEGK VRKEREYVIP KRNEIKENKT SVSAKFSAQE IKTGLKEVVT AVEEMTSKGK481PGQEVLEDDQ ENTLKYEYEE DFEVDEEKQG EKSNEEGQAD VQMNGIPQSP LDDKKDNLDP541EKESETSSQK APDARDNVKD ENDGCSESEL EEDKQDMKTA SSTSSRSHPY SSDSEDESAV601GDREAHTDSS TDESARRSSS QELSENDKPR KSHLPIEESL EIEIEDQEIT KADVETKPMP661IDESFENVLK EGTEKGTQEI AEGLSEKSGK HVSAEEKEKD KSKLWEESTA QVKDKKAGLP721GLEEGGKDSL PLAYVLALGA PTMNFMVDET AAINSNKESQ QLVQKTYTLE KKEAMEEDEA781PQHRDADIVQ GKGEAALWGE AGAVHEAPLR AWKPTAEQPE LAEEFTEKRE IPPGIERGAE841GAAEAEGVRR LGEGGSDPIG QAAAKDAVGL SKDEAPEKQA LMLTVLETDK AASEGEQGLE901KAVLANEAAA LNLEHLHEVA ALREAATSEE GEAEGGVAVS DVGESEEEAS IDLEDTGPME961DTASKREDGS EEAILGGEEP AKERKEVMRT ETRLSPFTGE AEASRMQVSE GSPEEGSLAK1021EAFLCKEDVE GEEMVTEAEA NREDDRKEIL PKELDLARER RKAERPKTSL RKTDSEREEV1081TRANALKDED AFKEEQKLKA EEGETETEVR AEEETKAPPN EMGSDAENEA PVEASELSDN1141PGLLGEDSLK ETVVPIFEAT PGFEKSLENI TALRKEGGGE RLSEARDTEH KDREELSSRE1201NRALKEGHRQ DGEGALAAPE AEPAGKVQAP EGLIPATGQA EELAAKDHDS CAGLEGRAEG1261QGGVDVVLRT QEAVAEEDPI MAEKFREEAV DEDPEEEEDK ECTLETEAMQ DRNSEGDGDM1321EGEGNTQKNE GMGGGRVVAV EVLHGGGETA ETAAEEREVL AGSETAEEKT IANKASSFSD1381VAEEETWHQQ DELVGKTAAA GKVVVEELAR SGEEVPAAEE MTVTYTTEAG VGTPGALERK1441TSGLGQEQEE GSEGQEAATG SGDGRQETGA AEKFRLGLSR EGERELSPES LQAMATLPVK1501PDFTETREKQ QHMVQGESET ADVSPNNMQVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1530 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.