Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERI1 / HEXO Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105781-100
Catalog Number: LS-G105781
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEXO(ERI1) (NM_153332), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 3 exoribonuclease | 3'HEXO | 3-5 exonuclease ERI1 | 3HEXO | 3-5 exoribonuclease 1 | 3EXO | Exoribonuclease 1 | ERI1 | Histone mRNA 3-exonuclease 1 | Protein 3hExo | THEX1 | Eri-1 homolog | HEXO | Three prime mRNA exonuclease 1
- Origin Species
- Human
- NCBI GenBank
- NM_153332
- SwissProt
- Q8IV48
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDPQSKEPA GEAVALALLE SPRPEGGEEP PRPSPEETQQ CKFDGQETKG SKFITSSASD61FSDPVYKEIA ITNGCINRMS KEELRAKLSE FKLETRGVKD VLKKRLKNYY KKQKLMLKES121NFADSYYDYI CIIDFEATCE EGNPPEFVHE IIEFPVVLLN THTLEIEDTF QQYVRPEINT181QLSDFCISLT GITQDQVDRA DTFPQVLKKV IDWMKLKELG TKYKYSLLTD GSWDMSKFLN241IQCQLSRLKY PPFAKKWINI RKSYGNFYKV PRSQTKLTIM LEKLGMDYDG RPHCGLDDSK301NIARIAVRML QDGCELRINE KMHAGQLMSV SSSLPIEGTP PPQMPHFRKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
349 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.