Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERG1 / SQLE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117539-100
Catalog Number: LS-G117539
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Squalene Epoxidase(SQLE) (NM_003129), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ERG1 | SQLE | Squalene monooxygenase | Squalene oxidase | SE | Squalene epoxidase
- Origin Species
- Human
- NCBI GenBank
- NM_003129
- SwissProt
- Q14534
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWTFLGIATF TYFYKKFGDF ITLANREVLL CVLVFLSLGL VLSYRCRHRN GGLLGRQQSG61SQFALFSDIL SGLPFIGFFW AKSPPESENK EQLEARRRRK GTNISETSLI GTAACTSTSS121QNDPEVIIVG AGVLGSALAA VLSRDGRKVT VIERDLKEPD RIVGEFLQPG GYHVLKDLGL181GDTVEGLDAQ VVNGYMIHDQ ESKSEVQIPY PLSENNQVQS GRAFHHGRFI MSLRKAAMAE241PNAKFIEGVV LQLLEEDDVV MGVQYKDKET GDIKELHAPL TVVADGLFSK FRKSLVSNKV301SVSSHFVGFL MKNAPQFKAN HAELILANPS PVLIYQISSS ETRVLVDIRG EMPRNLREYM361VEKIYPQIPD HLKEPFLEAT DNSHLRSMPA SFLPPSSVKK RGVLLLGDAY NMRHPLTGGG421MTVAFKDIKL WRKLLKGIPD LYDDAAIFEA KKSFYWARKT SHSFVVNILA QALYELFSAT481DDSLHQLRKA CFLYFKLGGE CVAGPVGLLS VLSPNPLVLI GHFFAVAIYA VYFCFKSEPW541ITKPRALLSS GAVLYKACSV IFPLIYSEMK YMVHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
574 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.