Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ERG-1 / CUZD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110530-100
Catalog Number: LS-G110530
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CUZD1 (NM_022034), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Estrogen regulated gene 1 | UO-44 | CUZD1 | ERG-1 | Transmembrane protein UO-44
- Origin Species
- Human
- NCBI GenBank
- NM_022034
- SwissProt
- Q86UP6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MELVRRLMPL TLLILSCLAE LTMAEAEGNA SCTVSLGGAN MAETHKAMIL QLNPSENCTW61TIERPENKSI RIIFSYVQLD PDGSCESENI KVFDGTSSNG PLLGQVCSKN DYVPVFESSS121STLTFQIVTD SARIQRTVFV FYYFFSPNIS IPNCGGYLDT LEGSFTSPNY PKPHPELAYC181VWHIQVEKDY KIKLNFKEIF LEIDKQCKFD FLAIYDGPST NSGLIGQVCG RVTPTFESSS241NSLTVVLSTD YANSYRGFSA SYTSIYAENI NTTSLTCSSD RMRVIISKSY LEAFNSNGNN301LQLKDPTCRP KLSNVVEFSV PLNGCGTIRK VEDQSITYTN IITFSASSTS EVITRQKQLQ361IIVKCEMGHN STVEIIYITE DDVIQSQNAL GKYNTSMALF ESNSFEKTIL ESPYYVDLNQ421TLFVQVSLHT SDPNLVVFLD TCRASPTSDF ASPTYDLIKS GCSRDETCKV YPLFGHYGRF481QFNAFKFLRS MSSVYLQCKV LICDSSDHQS RCNQGCVSRS KRDISSYKWK TDSIIGPIRL541KRDRSASGNS GFQHETHAEE TPNQPFNSVH LFSFMVLALN VVTVATITVR HFVNQRADYK601YQKLQNYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
607 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.