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ERCC6L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ERCC6L2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ERCC6L2 antibodies →
$725.00each
Catalog Number: LS-G121724-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAD26L(ERCC6L2) (NM_001010895), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C9orf102 | ERCC6L2 | RAD26L hypothetical protein | RAD26L | SR278 | Stretch responsive protein 278
Origin Species
Human
NCBI GenBank
NM_001010895

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MQPGSAPPPG RMDPSAPQPR AETSGKDIWH PGERCLAPSP DNGKLCEASI KSITVDENGK
61SFAVVLYADF QERKIPLKQL QEVKFVKDCP RNLIFDDEDL EKPYFPNRKF PSSSVAFKLS
121DNGDSIPYTI NRYLRDYQRE GTRFLYGHYI HGGGCILGDD MGLGKTVQVI SFLAAVLHKK
181GTREDIENNM PEFLLRSMKK EPLSSTAKKM FLIVAPLSVL YNWKDELDTW GYFRVTVLHG
241NRKDNELIRV KQRKCEIALT TYETLRLCLD ELNSLEWSAV IVDEAHRIKN PKARVTEVMK
301ALKCNVRIGL TGTILQNNMK ELWCVMDWAV PGLLGSGTYF KKQFSDPVEH GQRHTATKRE
361LATGRKAMQR LAKKMSGWFL RRTKTLIKDQ LPKKEDRMVY CSLTDFQKAV YQTVLETEDV
421TLILQSSEPC TCRSGQKRRN CCYKTNSHGE TVKTLYLSYL TVLQKVANHV ALLQAASTSK
481QQETLIKRIC DQVFSRFPDF VQKSKDAAFE TLSDPKYSGK MKVLQQLLNH CRKNRDKVLL
541FSFSTKLLDV LQQYCMASGL DYRRLDGSTK SEERLKIVKE FNSTQDVNIC LVSTMAGGLG
601LNFVGANVVV LFDPTWNPAN DLQAIDRAYR IGQCRDVKVL RLISLGTVEE IMYLRQIYKQ
661QLHCVVVGSE NAKRYFEAVQ GSKEHQGELF GIHNLFKFRS QGSCLTKDIL EV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

712 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.